Plasmid

Part:BBa_K112145:Design

Designed by: Madhvi Venkatesh   Group: iGEM08_UC_Berkeley   (2008-11-01)


Phagemid plasmid with xis int


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5295
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5295
    Illegal NotI site found at 4873
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5295
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5295
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5295
    Illegal AgeI site found at 2065
    Illegal AgeI site found at 2844
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2047


Design Notes

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page


Source

K112144 + K112132

References